Characterization of DCs, Mø1s, and Mø2s. (A) DCs, Mø1s, and Mø2s were generated in parallel from the same donor cultured for 6 or 7 days. Pictures show the morphology of these 3 different types of cells. Images were obtained using an Axiovert 25 inverted microscope (Carl Zeiss, Sliedrecht, The Netherlands) with a 20 × /0.3 NA objective and Zeiss Axiovision software version 3.1. Magnification, × 200. (B) Surface expression of CD1a, CD14, DC-SIGN, mannose receptor (MR), and CD11b on DCs, Mø1s, and Mø2s was determined by flow cytometry after 6 days of culture (closed histograms). Open histograms represent matched isotype controls. Data are representative of 3 to 8 independent experiments. Separate unrelated donors are used for each independent experiment.