Cytokine production by DCs, Mø1s, and Mø2s after LPS stimulation or uptake of apoptotic cells. (A-C) Day-6 DCs, Mø1s, and Mø2s were extensively washed, and subsequently stimulated with or without LPS for 24 hours. (D-F) DCs, Mø1s, or Mø2s (1 × 10⁵ cells) were cocultured with etoposide-induced apoptotic cells at a 1:1 ratio for 24 hours in RPMI culture medium. Supernatants were harvested and measured by ELISA for IL-10, IL-6, and TNF-α. Data are presented as mean ± SD from duplicate cultures and are representative of at least 3 independent experiments where cells are generated from separate unrelated donors. Dashed lines represent the detection limits of ELISA.