Uptake of viable, early apoptotic, late apoptotic, and necrotic cells by DCs, Mø1s, and Mø2s. (A) Early and late apoptotic cells were induced by UV-C light at a dose of 50 J/m2, then cultured for another 4 and 30 hours, respectively. Necrosis was induced by incubating Jurkat cells at 56°C for 1 hour. The untreated viable cells were used as control. Apoptosis was confirmed by double staining with FITC-labeled annexin V and PI. (B) These CFSE-labeled cells were cocultured with DCs, Mø1s, or Mø2s (1 × 105 cells) at a 1:1 ratio, in the same way as described in Figure 2. The percentages of uptake and binding (at 37°C) or binding (at 4°C) were quantified similarly as described in Figure 2. Data (mean ± SD) represent at least 3 independent experiments performed in duplicate. (C) Uptake of early apoptotic cells by Mø2s (mean ± SD) generated in the presence or absence of 10 μg/mL neutralizing anti–IL-10 receptor mAb.
