Lectin-mediated endocytosis and macropinocytosis by DCs, Mø1s, and Mø2s. (A) Mannose receptor (MR)/CD206 expression on DCs, Mø1s, and Mø2s. Bars show the mean fluorescence intensity (MFI). Data shown are the mean (± SD) from 2 to 3 independent experiments. (B) The uptake of Dextran^FITC (100 μg/mL) by DCs, Mø1s, and Mø2s was measured at 1 hour. Data shown are the mean ± SD from 3 independent experiments. (C) The uptake of Dextran^FITC was measured by different phagocytes preincubated with 100 μg/mL mannan, or 50 mM d-mannose or l-mannose. The uptake by untreated phagocytes (control) is considered as 100%, and data show the relative uptake against the control. Data represent 3 independent experiments. (D) The uptake of BSA^FITC (0.2 μg/mL) by DCs, Mø1s, and Mø2s at 1 hour. Data shown are the mean ± SD from 5 independent experiments. Dashed line indicates 100% of relative uptake. (E) The uptake of lucifer yellow (LY) (100 μg/mL) by DCs, Mø1s, and Mø2s at 1 hour. Data shown are the mean ± SD from 3 independent experiments (**P < .001, ANOVA).