Uptake of early apoptotic cells and apoptotic blebs by Mø2s is prevented by an inhibitor of macropinocytosis. Mø2s were preincubated with or without DMA at 37°C for 20 minutes before the coculture with BSA^FITC (0.2 μg/mL), LY (100 μg/mL), or early apoptotic cells. (A) Data show the uptake of BSAFITC and LY at 1 hour by Mø2s in the presence (gray histograms) or absence (black histograms) of 500 μM DMA. The dashed lines represent the background mean fluorescence of Mø2s. (B) Data show the dose-dependent effect of DMA on uptake (37-4°C) of apoptotic cells and LY by Mø2s and represent the relative uptake of DMA-treated Mø2s against the controls (untreated cells). Data are shown as mean ± SD of duplicate cultures and represent 4 independent experiments. (C) Mø1s were treated with or without 500 μM DMA at 37°C for 20 minutes before the co-incubation with early apoptotic cells for 30 minutes. Data show the quantification of uptake (37-4°C) by CD14^high and CD14^low cells. Data (mean ± SD) represent 3 independent experiments. (D) Apoptotic blebs were isolated from CFSE-labeled Jurkat cells and were used for the phagocytosis assay with Mø1s and Mø2s. Data (mean ± SD) represent 4 independent experiments. (E) Mø2s were pretreated with or without DMA (up to 500 μM). Relative uptake is shown. Similar results were obtained from 2 independent experiments. Separate unrelated donors are used for each independent experiment.