Conditional activation of Akt in A20 B cells increases glucose utilization. (A) A20 cells constitutively expressing an Akt-mER fusion protein were cultured in the absence or presence of 4 μM 4-HT or 10 μg/mL anti-Ig (αIg) for 5 and 30 minutes. Cells were then harvested, and phosphorylation of Akt-mER (Myr Akt-ER) on Ser473 was monitored by Western blot. A20 cells constitutively expressing an Akt-mER fusion protein were cultured in the absence or presence of 4 μM 4-HT for 24 hours, and then uptake of 2-[³H]deoxyglucose (B) and glycolysis (C) was measured as described in “Materials and methods.” In panels B-C, error bars reflect standard deviation from the mean of triplicate measurements.