A1 mRNA is up-regulated by WT1 (–Ex5/–KTS). (A) Semiquantitative RT-PCR was performed as described on RNA isolated from control 32DV4 cells and from 32DWT1 and 32DWT2 cells treated overnight with (+) and without (–) 50 μM ZnCl2. Arrows indicate the Actin (control) and A1 PCR products. MW indicates the position of the molecular-weight markers. (B) RT-PCR was performed as described on RNA isolated from U937 cells transduced with the empty MIGR-1 retroviral vector (lane 2) or the same vector with the WT1 (–Ex5/–KTS) cDNA (lane 1). A water control is in lane 3. The arrow indicates the position of the WT1 band. (C) RT-PCR was performed on the same samples using A1 primers. The arrow indicates the position of the A1 band. (D) RT-PCR was performed on the same samples using 36B4 primers.
