Significant increase in the percentage and absolute number of KLSCD48^– cells in SHIP^–/– BM. (A) Representative FACS plots showing detection of KLSCD48^– HSCs in SHIP^–/– and WT BM. (B) Percentage and absolute number of KLSCD48^– cells in the BM (per femur and tibia pair) and spleen (Spl) of SHIP^–/– (▪) and WT (▴) mice. (C) Histogram of DNA content in SHIP^–/– and WT KTLS cells. (D) Bar graph showing the percentage of SHIP^–/– (▪) or WT (▦) KTLS/KFLS cells in each stage of cell cycle as calculated using the Watson Pragmatic model in the FlowJo cell-cycle platform. Statistical analysis was performed using Prism 4. Significance was established using the unpaired student t test (Prism 4; GraphPad Software, San Diego, CA). **P < .001 and *P < .01 (mean ± SEM; n ≥ 3).