SHIP^–/–Sca1⁺Lin^– cells do not home to the BM as efficiently as WT Sca1⁺Lin^– cells. (A) Representative FACS plot of BM from transplant recipients after gating on live cells. (Ai) BM from control recipient 12 to 14 hours after transplantation with 0.5 × 10⁶ WBM stained with 1 μM DDAO. (Aii) BM from control recipient 12 to 14 hours after transplantation with 0.5 × 10⁶ WBM stained with 0.5 μM CFSE. (Aiii) Representative FACS plot of BM from a mouse 12 to 14 hours after transplantation with 2 × 10⁵ SHIP^–/–Lin^–Sca1⁺ cells stained with 0.5 μM CFSE and 2 × 10⁵ WT Lin^–Sca1⁺ cells stained with 1 μM DDAO. (B) Left panel shows percentage of dye⁺ SHIP^–/– (▪) or WT(▦) Sca1⁺Lin^– cells found in the recipient BM 12 to 14 hours after transplantation. Right panel shows percentage of SHIP^–/– (▪) or WT (▦) Sca1⁺Lin^– cells that trafficked to and were retained in BM of recipients over the total number of cells injected, 12 to 14 hours after transplantation. (C) Left panel shows percentage of stained SHIP^–/– (▪) or WT (▦) Sca1⁺Lin^– cells found in the spleen of recipients 12 to 14 hours after transplantation. Right panel shows percentage of SHIP^–/– (▪) or WT (▦) Sca1⁺Lin^– cells that reached the spleen of recipients over the total number of cells injected, 12 to 14 hours after transplantation. Significance was established using the stratified Wilcoxon-Mann Whitney test using StatXact (Cytel Software, Cambridge, MA): ⁺P < .05; *P < .01; and ⁺⁺P < .005 (mean ± SEM; n = 12).