Figure 5.
![Figure 5. SHIP–/–HSCs express lower levels of CXCR4 and VCAM-1 molecules as assessed by flow cytometry. (A) A representative histogram of CXCR4 (left) and VCAM-1 (right) expression on live (DAPI-) KTLS cells (isotype control [□]), SHIP–/– [▪], and WT [▦]). (B) The plots on the left represent the mean fluorescence intensity (MFI) of KTLS cells for CXCR4, VCAM-1, and VLA-4, respectively, and the plots on the right show the percentage of KTLS cells positive for VCAM-1 and VLA-4, respectively (SHIP–/– [▪], WT [▴]). Significance was established using the unpaired student t test (Prism 4): **P < .001 and +P < .05 (mean ± SEM; n ≥ 3).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/107/11/10.1182_blood-2005-12-5021/4/zh80110696400005.jpeg?Expires=1735513430&Signature=dEP8ovoI-~vgRYbR-zh8gvti4V38Gs~HKk3cY6zw0xWtEArCX-k2ILMdPzRM75Y2E1sun6a1brZ1RL~PAUfSRYsL8gg0qqojsj2meRnfifos-npAtxTMQg3PlR83rvOC1YhZ~Gc7yoEC~HcDq254TmWscCaETPLnQ30OE61Q5FUFxHjU3c1jdPr-Vz1vZOPBYA8dM2hgDOUdtzs~2aU~278Yv0XW7YbkmevfK4UN8-LtSYXDF41F9LbHw4sv1OUM5gI601~etn89L~t5h1N6NxN0I6zPCgd9p-he3Jrz7D~U8PDxqvlrZ4QuUEBU~aw~GliaCw8quhLUCnRclso9cQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
SHIP–/–HSCs express lower levels of CXCR4 and VCAM-1 molecules as assessed by flow cytometry. (A) A representative histogram of CXCR4 (left) and VCAM-1 (right) expression on live (DAPI-) KTLS cells (isotype control [□]), SHIP–/– [▪], and WT [▦]). (B) The plots on the left represent the mean fluorescence intensity (MFI) of KTLS cells for CXCR4, VCAM-1, and VLA-4, respectively, and the plots on the right show the percentage of KTLS cells positive for VCAM-1 and VLA-4, respectively (SHIP–/– [▪], WT [▴]). Significance was established using the unpaired student t test (Prism 4): **P < .001 and +P < .05 (mean ± SEM; n ≥ 3).
