Figure 6.
![Figure 6. Effect of the plasminogen active site mutant, [D(646)E]-Plg, on apoptosis. U937 cells were either untreated (diamonds) or exposed to 10 ng/mL TNFα (squares) (A) or 50 μg/mL CHX (circles) (B) in the absence or presence of increasing concentrations of either the plasminogen active site mutant, [D(646)E]-Plg (closed symbols), or wild-type plasminogen (open symbols) for 24 hours at 37°C. The cells were analyzed by FACS with annexin V–FITC and PI. Data are shown as mean ± SE, n = 2, for a representative of 3 independent experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/107/11/10.1182_blood-2005-07-2872/4/zh80110696210006.jpeg?Expires=1735562798&Signature=I3xtjHU3ZSBFisylwJrTST1br4ThPmPv25lJ8YLLtO1gqEF3869gIvKX626~tH5pBtgIHWUwpFKdZbRgy-8e4yM8ylGojnjO8kKROJ8Dr5Rqp4kMvM-tFX2zedhxZnaS7dVy7MxDeTaKjUOhCbtqtPeUUylXXg8kPbPN7HYyb3TOHiV9gTcDjIyF4HbtFCgG7dLJ8SZ7ZxUQwKPKPI9LlOkefx0jrg~axNx3t0~8ZQ21twLfM3iWNl0sOCVjy1QELR0F6OVIVkEi738eijzgz87tpqtIiNNMyWO9g9puh0tSx1XWhd4Wqvltqknzqi1nyC85rA-u~PhPjuefDZ~2bQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of the plasminogen active site mutant, [D(646)E]-Plg, on apoptosis. U937 cells were either untreated (diamonds) or exposed to 10 ng/mL TNFα (squares) (A) or 50 μg/mL CHX (circles) (B) in the absence or presence of increasing concentrations of either the plasminogen active site mutant, [D(646)E]-Plg (closed symbols), or wild-type plasminogen (open symbols) for 24 hours at 37°C. The cells were analyzed by FACS with annexin V–FITC and PI. Data are shown as mean ± SE, n = 2, for a representative of 3 independent experiments.
