Fli-1, Erg, and Elf-1 bind the –8 enhancer and the promoter region of endoglin in MS1 cells but not NIH3T3 cells. (A) The expression profiles of selected Ets and GATA factors and their enrichment at E1-E3 segments in endoglin expressing MS1 endothelial cells. (Ai) Expression analysis by RT-PCR. (Aii) Chromatin immunoprecipitation assays were performed with anti-Ets1, anti-Ets2, anti–Fli-1, anti-Erg, anti–Elf-1, anti-GATA2, and control immunoglobulin G (IgG) antibodies. The DNA content of the immunoprecipitates was analyzed by real-time PCR for the conserved segments E1-E3. The level of enrichment with each antibody was normalized to the levels obtained with the control IgG and plotted as a fold increase over the level of enrichment at a control region (3′ UTR of endoglin). Fli-1, Erg, and Elf-1 are significantly enriched at E1 and E3 but not E2. (B) The expression profiles of selected Ets and GATA factors and their enrichment at E1-E3 segments in endoglin nonexpressing NIH3T3 fibroblasts. (Bi) RT-PCR expression analysis. Erg is not expressed in NIH3T3 cells. (Bii) Chromatin immunoprecipitation assay. None of the Ets and GATA factors were bound to E1-E3 segments in NIH3T3 cells. Error bars indicate standard deviation.