Macroscopic and microscopic analyses of the spleen of SHPS-1 mutant mice. (A) Spleens of WT and homozygous SHPS-1 mutant (KO) mice at 7 weeks of age. (B) Weight of the spleen of WT and SHPS-1 mutant mice at 6 or 13 weeks of age. Data are means ± SE of values from 5 mice of each group. ^*P < .05. (C) The spleen of WT or SHPS-1 mutant mice at 6 weeks of age was fixed and stained with Mayer hematoxylin-eosin. Data are representative of 3 independent experiments. Scale bar, 0.5 mm. Images were visualized using a BX50 microscope equipped with a 4×/0.16 objective lens (Olympus), captured with a DP70 camera (Olympus) and analyzed with Adobe Photoshop 8.0 software (Adobe Systems). (D) The numbers of RBCs and mononuclear cells (MNCs) in the spleen of WT or SHPS-1 mutant mice at 6 weeks of age were counted. Data are means ± SE of values from 5 mice of each genotype. NS indicates not significant; ^*P < .05.