American Society of Hematology

Figure 4.

$Figure 4. Lifespan of circulating RBCs and accumulation of hemosiderin in the spleen of SHPS-1 mutant mice. (A) WT (○) or homozygous SHPS-1 mutant (•) mice were injected intravenously with CFSE-labeled RBCs derived from WT (left) or homozygous SHPS-1 mutant (right) mice. Blood samples (5 μL) were collected from a tail vein at the indicated times thereafter, and the fraction of fluorescent RBCs among recipient RBCs (100 000 per sample) was determined by flow cytometry. Data are expressed as a percentage of the value determined 5 minutes after injection and are means ± SE of values from 5 recipients per group. The percentage of CFSE-positive RBCs at 5 minutes after injection was 7% to 10% of total RBCs determined. (B) Spleen sections from WT or SHPS-1 mutant mice at 6 weeks of age were fixed with paraformaldehyde and stained with Berlin blue. Scale bar, 0.5 mm. Image acquisition performed as described for Figure 2C.$

Lifespan of circulating RBCs and accumulation of hemosiderin in the spleen of SHPS-1 mutant mice. (A) WT (○) or homozygous SHPS-1 mutant (•) mice were injected intravenously with CFSE-labeled RBCs derived from WT (left) or homozygous SHPS-1 mutant (right) mice. Blood samples (5 μL) were collected from a tail vein at the indicated times thereafter, and the fraction of fluorescent RBCs among recipient RBCs (100 000 per sample) was determined by flow cytometry. Data are expressed as a percentage of the value determined 5 minutes after injection and are means ± SE of values from 5 recipients per group. The percentage of CFSE-positive RBCs at 5 minutes after injection was 7% to 10% of total RBCs determined. (B) Spleen sections from WT or SHPS-1 mutant mice at 6 weeks of age were fixed with paraformaldehyde and stained with Berlin blue. Scale bar, 0.5 mm. Image acquisition performed as described for Figure 2C.

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