Lyn protein depletion has no effect on cell proliferation and viability. (A) Cells were tested at 48 to 72 hours after electroporation with Lyn or control siRNA for their ability to proliferate and survive in medium with 10% FCS by MTT assay. Values are mean plus or minus SD (n = 4). (B) Functional rescue experiment. Chemotaxis assays (n = 3) in Mo7e cells were performed at 48 hours after transfection with the Lyn siRNA targeting the 3′ UTR and cotransfection with a Lyn expression construct lacking the 3′ UTR. Bottom blot represents control Western blots in these cells.