Interferon- and LPS-induced Ttp expression has a suppressive effect on Ccl2 and Ccl3 mRNA production. BMMs derived from TTP wild-type (TTPWT) and deficient (TTPKO) mice were left untreated or treated with IFN-γ(g), LPS (L), or both (L/g) for 3 hours. Total RNA was extracted and the induction of Ccl2 (A), Ccl3 (B), Gbp2 (C), and Cxcl10 (D) genes was detected by real-time RT-PCR using Taqman assays from Applied Biosystems. To obtain mRNA induction, qRT-PCR values were normalized to those of untreated cells. *P < .05, treated versus untreated cells by univariate linear regression models.