Lyn inhibition reduces fMLP-induced migration and increases cell adhesion. HL-60 cells were differentiated for 3 days with 1.25% DMSO into neutrophil-like cells. Prior to stimulation, cells were pretreated for 2 hours with a Lyn-specific peptide (1 μM) or control buffer. HL-60 cells were then stimulated in the presence or absence of peptide with fMLP (100 nM) or SDF-1 (100 ng/mL) and a chemotaxis (left panel) or adhesion assay on ICAM-1–coated plates (right panel) was performed. Data are representative of 3 independent experiments done in triplicate.