Activation of primary B cells leads to SNK/PLK2 gene expression. Primary B cells were treated with CD40 ligand or PMA, or infected with immortalizing strains of EBV as shown. Expression of Snk/Plk2 and the control gene ARPO was analyzed using RT-PCR and real time RT-PCR at different time points after activation/infection as described in “Materials and methods.” (A) RT-PCR showing mRNA expression of Snk/Plk2 and ARPO. Time points are shown above the gel. The top panel shows infection with EBV, the middle panel shows treatment with CD40 ligand, and the bottom panel shows treatment with PMA. (B) Real-time PCR analysis showing expression of Snk/Plk2 mRNA. ARPO was used as an internal control. The figure shows fold induction of Snk/Plk2 corrected for levels of the control gene ARPO (± 1 SD). Values obtained are derived from 3 independent experiments. Lanes are as indicated in brackets in panel A.