C/EBPα promotes myeloid development versus erythroid development in vivo. Femoral BMCs were prepared from 8-week-old inbred female C57Bl/6 mice that had been treated with 5-fluorouracil (5-FU) 4 days earlier to enrich for hematopoietic stem cells, and then infected with retrovirus containing MSCV-GFP or MSCV-C/EBPα-GFP. GFP+ BM cells (1 × 106) were transplanted into lethally irradiated mice with host bone marrow cells (2 × 105) to promote radioprotection. Four months after transplantation, the presence of donor-derived (GFP+) hematopoietic cells was determined by flow cytometry. Granulocytes and macrophages (Gr-1 and Mac-1) and erythroid cells (CD71 and TER119) in peripheral blood are presented in panels A-B, while myeloid (Mac-1) and erythroid (CD71 and TER119) cells in bone marrow are presented in panels C-D. These flow cytometry data were collected from 3 different transplantation experiments. (B, D) Horizontal bars indicate median values.
