CD16/γ vector design and expression in Jurkat cells. (A) Schematic representation of the chimeric Fc-γRIIIa-FcϵRIγ molecule. The CD16/γ chimeric cDNA comprised the leader (L) and the extracellular (EC) domain of CD16 (FcγRIIIa-158V allotype), 2 amino acids (aa) of the extracellular domain of the FcϵRIγ, as well as the intact transmembrane (TM) and intracellular (IC) domains. (B) Maintenance of chimeric receptor expression in Jurkat cells transduced with 20 μLof lentiviral virus stock. Transduced cells were analyzed by flow cytometry for CD16 expression over a 3-month period. Mean fluorescence intensities are indicated in each quadrant.