Detection of HIV-1 replication in iMDDCs. (A) Kinetics of iMDDC infection with purified HIV-1_BaL (2 μg/mL) were performed under experimental conditions described for the neutralization assay and 10 μM AZT was added at different times (0, 24, and 48 hours) to the cultures. The percentage of infected MDDCs was assessed by measurement of intracellular p24 antigen at different times after infection (up to 120 hours). Results are expressed as mean ± SD of triplicate values. (B) Dose-response between virus input and the percentage of iMDDCs positive for intracellular p24 viral antigen at 64 hours. Linear regression lines and r² for each HIV-1 primary isolate are represented. Data are from 1 representative experiment of 4 performed independently. (C) Dot plot representation of purified iMDDCs or iMDDCs cocultured with 15% of autologous PBLs. Cells positive for intracellular p24 were detected 64 hours after HIV-1_BaL infection in the presence or in the absence of mAb 2F5 at 5 μg/mL. Percentages of infected iMDDCs and autologous PBLs from total cell population are indicated in each box.