Effect of monoclonal and polyclonal antibodies on MDDC maturation. CD83 expression was determined on iMDDCs 64 hours after addition of antibodies in the presence (□) or in the absence () of HIV-1_BaL. Abs were added at concentrations that resulted in 90% inhibition of infection (IC₉₀) (A) or at 5- to 25-fold higher concentrations (B). Values are from one representative experiment, repeated 4 times with iMDDCs from different HIV-negative donors. In A and B, □ indicates uninfected MDDC; , HIV-1_BaL-infected.