Detection of DMT1 protein in PBMCs. Total PBMC extracts were prepared as described: 80 μg total protein was loaded into each lane and was separated by SDS-PAGE on a 10% acrylamide gel followed by transfer to PVDF membranes. Membranes were incubated with anti-DMT1-NT antibody (A) and with β-actin antibody as an internal loading control (B). Samples in the different lanes are indicated. Sizes (in kDa) are on the right.