Figure 2.
Figure 2. Rescue of primitive erythropoiesis by conditional expression of GATA-1. (A) May-Giemsa and benzidine staining of EryPs differentiated from control ES cells and TET-G1KO cells at day 8 of induction. TET-G1KO cells were cultured in the presence (100 ng/mL) or absence of tetracycline from day 5. (B) FACS analysis of TER-119 and CD71 of the day-8 EryPs. TET-G1KO cells were cultured in the presence (100 ng/mL) or absence of tetracycline from day 5. (C-D) Number of the cells and percentage of benzidine-positive cells at day 8 of induction. Control ES cells and TET-G1KO cells were cultured with medium containing tetracycline as shown. Data are means ± SD of 6 samples. (E) Western blot of GATA-1 at day 8 of induction. TET-G1KO cells were cultured in the media containing tetracycline as indicated.

Rescue of primitive erythropoiesis by conditional expression of GATA-1. (A) May-Giemsa and benzidine staining of EryPs differentiated from control ES cells and TET-G1KO cells at day 8 of induction. TET-G1KO cells were cultured in the presence (100 ng/mL) or absence of tetracycline from day 5. (B) FACS analysis of TER-119 and CD71 of the day-8 EryPs. TET-G1KO cells were cultured in the presence (100 ng/mL) or absence of tetracycline from day 5. (C-D) Number of the cells and percentage of benzidine-positive cells at day 8 of induction. Control ES cells and TET-G1KO cells were cultured with medium containing tetracycline as shown. Data are means ± SD of 6 samples. (E) Western blot of GATA-1 at day 8 of induction. TET-G1KO cells were cultured in the media containing tetracycline as indicated.

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