American Society of Hematology

Figure 3.

$Figure 3. Effects of GATA-1 on primitive erythropoiesis. (A) Number of cells at day 8 of induction after the conditional expression of GATA-1 for the indicated periods. Data are means ± SD of 6 samples. The numbers of cells showed no significant differences among those of D5-6, D5-7, and D5-8. (B) Percentage of benzidine-positive cells at day 8 of induction after the conditional expression of GATA-1 for the indicated periods. Data are means ± SD of 6 samples. The data show significant intervalue differences (P < .01 by Student t test). (C) FACS analysis of TER-119 and CD71 on day 8 induced cells after the conditional expression of GATA-1 for the indicated periods. Ovals demonstrate the fraction of TER119+CD71hi cells, and the percentages of the cells in the fraction are indicated. (D) Western blot of GATA-1 at day 8 after the conditional expression of GATA-1 for the indicated periods.$

Effects of GATA-1 on primitive erythropoiesis. (A) Number of cells at day 8 of induction after the conditional expression of GATA-1 for the indicated periods. Data are means ± SD of 6 samples. The numbers of cells showed no significant differences among those of D5-6, D5-7, and D5-8. (B) Percentage of benzidine-positive cells at day 8 of induction after the conditional expression of GATA-1 for the indicated periods. Data are means ± SD of 6 samples. The data show significant intervalue differences (P < .01 by Student t test). (C) FACS analysis of TER-119 and CD71 on day 8 induced cells after the conditional expression of GATA-1 for the indicated periods. Ovals demonstrate the fraction of TER119⁺CD71^hi cells, and the percentages of the cells in the fraction are indicated. (D) Western blot of GATA-1 at day 8 after the conditional expression of GATA-1 for the indicated periods.

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