EBNA2 enhances FcRH5 protein levels. EBNA2 activity was induced in BJAB-K3 and BL41-K3 cells with estrogen for 6 to 48 hours, as indicated. (A) Proteins were purified and analyzed by Western blotting using FcRH5 and actin-specific Ab. (B) Membrane FcRH5 protein expression was analyzed by flow cytometry using FcRH5 specific Ab and isotypic control Ab. In each panel, the 24-hour samples are shown as a thick line. Representatives of 3 experiments are shown.