EBNA2 induces FcRH5 through CBF1, independent of new protein synthesis. (A) BJAB-K3 cells were treated in one of the following ways; with estrogen (E), with cyclohexamide, a protein synthesis inhibitor (CHX), or with both estrogen and cyclohexamide (E + CHX). RNA samples were collected before treatment (time 0), and 12 hours later. cDNA were synthesized and used as template in quantitative real-time PCR with FcRH5- and CD21-specific primers. Fold increases in mRNA content at 12 hours relative to the time 0 samples are shown. Mean and SEM of 3 independent experiments are shown. (B) EBNA2 activity was induced in wild-type (WT) and CBF1^–/– DG75 cells with estrogen for 6 to 48 hours, as indicated. RNA samples were collected and cDNA were synthesized and used as template in quantitative real-time PCR with FcRH5 specific primers. Fold increases in mRNA content relative to the time 0 sample of the wild-type DG75 cells are shown. Mean and SEM of 2 independent experiments are shown.