EBNA2 targets the endogenous FcRH5 promoter. BJAB-K3 cells that had been treated with estrogen for 5 hours to induce EBNA2, or left untreated, were crosslinked with formaldehyde. Soluble, fragmented chromatin was immunoprecipitated with (A) EBNA2, (B) CBF1, or (C) hnRNP D Ab, or without Ab. Precipitated DNA were used in quantitative real-time PCR as template. Fold increases in DNA content relative to the no-Ab controls are shown. ▦ denotes uninduced samples; ▪, samples with induced EBNA2. At the bottom is a schematic representation of the FcRH5 gene from approximately –2000 to +200, relative to the transcription start site. Potential CBF1 () and hnRNP D (○) binding sites and the likely TATA box (★) are marked. Inverted arrows indicate the positions of PCR primer pairs (see Table 1 for primer sequences). At the right of each panel, binding of proteins to the known EBNA2-responsive CBF1 binding site at –207 (see Table 2) of the CD23 promoter is shown.