Noxa RNAi decreases response to bortezomib. (A) Noxa siRNA and nonsilencing-siRNA were transferred to Jeko and Jurkat cells by electroporation as described in “Patients, materials, and methods.” Total RNA was isolated 6 hours after transfection. Noxa mRNA levels were determined by quantitative RT-PCR (Taqman technology) using GUS as housekeeping gene. The results showed are the mean of 2 different experiments. (B) Jeko and Jurkat cells, transfected with nonsilencing siRNA (ns-siRNA) and with Noxa siRNA (Noxa-siRNA), were treated with 20 nM bortezomib for 18 hours. Viability was assessed by DiOC6(3) staining and FACs analysis. The results showed are the mean of 2 different experiments. In the same conditions, Noxa protein levels were also analyzed by Western blotting.
