Figure 3.
![Figure 3. Effect of various compounds on GC-box binding. EMSA with [32P]-labeled GC-box oligonucleotide was performed as follows: nuclear proteins (6 μg) from parental cells and their antifolate-resistant subline before and after treatment with Bt2-cAMP, IBMX, or pCPT-cGMP (A), pCPT-cGMP, pCPT-cGMP plus okadaic acid, or okadaic acid alone (B), were first incubated with [32P]-labeled GC-oligonucleotide, resolved by electrophoresis on polyacrylamide gels, and analyzed by a Phosphorimager.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/107/2/10.1182_blood-2005-07-2743/4/zh80020689610003.jpeg?Expires=1735584867&Signature=vSwjLT0c6rsUUMqNZR6E13uRZ6InFWPgAfvHpgDFp--uyvZThVzZv8cBQO8pp3MMG626ltxhdanJufsgUcdjYjP6OtVNCauoEIGVUckO7VlZq7pSSJ2ZHy7SWAdBHPUJ4ORbDsngBt~K23e9VeisiNzAI68JhKSWnFKLwIWLnvr2KwDToue-r9wUZwWqvVbpFBaKI0Hc4XY01PrTmYVhaLuIKUHzdVUfZ0E7tgEuo18K6gtRMEb6hlwIb1YrEVJOXeiQ8eWXkWFQkh9HyMIBMoenLjHCfoiWKQFgAxPdZjc8haj97GNxQDvGNCzpaXjNt-H7uKzxW48KnRdiW26o7w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of various compounds on GC-box binding. EMSA with [32P]-labeled GC-box oligonucleotide was performed as follows: nuclear proteins (6 μg) from parental cells and their antifolate-resistant subline before and after treatment with Bt2-cAMP, IBMX, or pCPT-cGMP (A), pCPT-cGMP, pCPT-cGMP plus okadaic acid, or okadaic acid alone (B), were first incubated with [32P]-labeled GC-oligonucleotide, resolved by electrophoresis on polyacrylamide gels, and analyzed by a Phosphorimager.
