Kinetics analysis of T gondii dissemination in C57BL/6 mice. Parasites were tracked using quantitative real-time PCR. Number of parasites per intestine, PP, MLN, and brain. The small intestine was cut into 4 equal-size segments (S), the S1 and S4 being proximal and distal to the stomach, respectively. The dot line represents the cut-off of sensitivity for the assay in the brain. Number of parasites per 100 μLof blood were estimated. Values represent mean number plus SD for 4 mice per group and 2 separate experiments.