Activation of NF-AT by antigen and inhibitory effects of cyclosporine-A on NF-AT activation and production of cytokines. IgE-primed MC/9 cells were stimulated or not (NS) in complete growth medium for 30 minutes for measurement of NF-AT oligonucleotide-binding activity (A) or for 3 hours for measurement of TNFα (B) and IL-6 (C). Stimulants included 100 ng/mL LPS, 1 μg/mL P3C, and 20 ng/mL antigen (Ag), alone or in combination. Where indicated, cultures were incubated with 1 μM cyclosporine A (CsA) for 60 minutes before addition of stimulants. The values are the mean ± SEM of 3 cultures. Identical results were obtained in 2 other experiments.