MET expression and missense germ-line MET mutations in DLBCL. (A) MET expression in normal lymphoid tissue and primary DLBCL. Immunohistochemical double staining of tonsillar sections for MET (blue) and IgD (brown), showing MET expression on germinal center B cells (left panel). Frozen tissue sections of DLBCL were stained with hematoxylin-eosin (HE) (middle panel) or with hematoxylin and anti-MET (brown, right panel). Image magnification: × 200. (B) Sequence analysis showing mutational transitions. PCR products were excised, reamplified, cloned, and sequenced. Shown are wild-type (WT) and mutant sequences. Mutational transitions are boxed.