HGFA expression in DLBCL cells. (A) Expression of HGFA in GC B cells and primary DLBCLs at mRNA level. After RNA isolation and cDNA synthesis, RT-PCR for MET was performed. β2-Microglobulin was used as housekeeping gene control. (B) mRNA expression of HGFA in DLBCL cell lines. (C) HGFA protein is expressed in DLBCL cell lines, but not in GC B cells. Cell lysates were immunoblotted using a monoclonal anti-HGFA antibody (A-1). MM cell line LME-1 was used as positive control. β-Actin was used as loading control. (D) Expression of HGFA protein in DLBCL lines and tonsillar B cells by immunocytochemical staining. DLBCL and tonsillar B cells were immunocytochemically stained with mAb A-1 against HGFA (A-1), CD20 (L26), or isotype control, as indicated. Image magnification: × 400.