Gene expression and cell-surface phenotype analyses of G1ME cells. (A) RT-PCR analysis. Bone marrow (BMarrow), spleen, and thymus were analyzed in parallel as controls. GM-CSFR indicates granulocyte macrophage colony-stimulating factor receptor; G-CSFR, granulocyte colony-stimulating factor receptor; Hprt, hypoxanthine phosphoribosyl transferase. PCR cycle numbers for each primer pair are shown at the right side of the panel. (B) Cell-surface marker phenotype assessed by flow cytometry. Antibody-stained cells are shown in filled curve. Open curves denote staining with appropriate isotype control antibodies. Murine bone marrow and/or thymus were used as positive controls (not shown).