Flow cytometric analysis of BM and FL from Lyl-1^–/–, lyl-1^+/–, and wild-type mice. (A) Analysis of the Lin^– Sca-1⁺ c-Kit⁺ population in the bone marrow of Lyl-1^+/+ (i), Lyl-1^+/– (ii), and Lyl-1^–/– (iii) mice and in the E14 FL from Lyl-1^+/+ (iv) and Lyl-1^–/– (iv) mice. (B) Proportions of CD34^– cells in the LSK population of BM from Lyl-1^+/+, Lyl-1^+/–, and Lyl-1^–/– mice. Graphs represent mean ± SEM of 3 independent experiments (*P < .05). (C) Analysis of side population in the total BM of Lyl-1^+/+ (i) and Lyl-1^–/– (ii) mice. BM cells were stained with Hoechst 33342. Side population was also quantified in the Lin^– cell population. BM cells from Lyl-1^+/+ (iii) and Lyl-1^–/– (iv) mice were stained with Hoechst 33342 and with mAb against cell surface markers. Flow cytometric profiles are representative of 3 independent experiments.