Analysis of the donor cell contribution after secondary transplantation of BM cells from primary animals grafted with Lyl-1^+/+ or Lyl-1^–/– BM cells. (A) Analysis of chimerism measured by the percentage of CD45.2⁺ cells in the blood after secondary transplantation. Secondary Ly5.1 recipient mice were injected with the same ratio as in primary graft (wt-5, wt-1, wt-0.5, KO-5, KO-1, and KO-0.5) of sorted Ly5.2 primary BM cells, and global chimerism was analyzed in PB 4 and 24 weeks after secondary transplantation. (B) The contribution of donor-derived cells to each reconstituted lineage is determined by analyzing the percentage of CD45.2⁺, B220⁺; CD45.2⁺, CD3⁺; and CD45.2⁺, GR-1/MAC-1⁺ cells in the PB of secondary recipients. Data represent mean ± SEM of 2 independent experiments (*P < .05).