Cell cycle analysis of Lin^– and LSK cell population from BM and E14 FL of Lyl-1^–/– mice. (A) Cell cycle of BM cells was analyzed with DNA dye Hoechst (Ho) and RNA dye pyronin (PY). Lin^– cells were sorted and stained with Ho and PY and then were stained with anti–Sca-1–FITC and anti–c-Kit–APC. Shown are representative flow cytometric profiles of 5 independent experiments. (B) Cell cycle of E14 FL cells was analyzed using BrdU dye. Lin^– and LSK cells were sorted and stained with BrdU for 1 hour (i-iv) and 48 hours (v-viii). Three independent experiments were performed with 3 mice per genotype.