Gene expression profiling of Lenti-KLF2– and Lenti-mock–transduced HUVECs. Microarray dye-swap hybridization analysis was performed comparing the gene expression profiles of 5 independent HUVEC isolates that were analyzed at 7 days after transduction with the Lenti-KLF2 virus and Lenti-mock virus. The composite plot of the 10 hybridizations is shown in panel A. The Loessnormalized weighted average hybridization signal intensity on the horizontal axis is plotted against the 10-base log of the ratio of Lenti-KLF2 over Lenti-mock. The genes with a statistically significant fold change (P < .01; according to the Rossetta Resolver error models and statistical evaluation) are shown in red (induced by KLF2) and green (repressed by KLF2) with error bars derived from the Rosetta Resolver error model. (B) Real-time semiquantitative RT-PCR verification of the microarray data on a selection of the genes presented in Tables 1 and 2. The RT-PCR was performed on the same cDNA preparations that were used for the microarray hybridization (n = 5). The data are represented as the mean ± SEM fold induction or fold repression compared with the Lenti-mock controls (▪). Also, the fold change in expression derived from the microarray data (□) is included for comparison.