Immunofluorescence analysis of typical endothelial marker genes that are under transcriptional control of KLF2. Immunofluorescence for TM (A-B), eNOS (C-D), and VWF (E-F) was performed on HUVEC cultures 7 days after transduction with mock (A,C,E) and KLF2 (B,D,F) lentivirus. Detergent permeabilization was omitted for TM staining, establishing a fully surface-exposed antibody-accessible localization. Expression of induced eNOS is predominantly located at the plasma membrane near intercellular junctions as also observed in sheared cultures compared with more Golgi-localized protein in mock-transduced cells. Images were acquired using a 10 ×/0.3 NA objective.