KLF2 overcomes the inflammatory TNF-α effect on the expression of hemostatic genes. HUVECs transduced with mock and KLF2 lentivirus for 7 days were either unstimulated or stimulated with TNF-α for 6 hours. (A) TM, eNOS, and PAI-1 protein levels were determined by Western blotting using α-tubulin as equal loading control. Two independent experiments are shown for the KLF2 overexpression with and without TNF-α. (B) Using a chromogenic assay, the effect of the induction of TM expression by KLF2 on the formation of active protein C (PC) was studied in the absence or presence of TNF-α for 6 hours. The amount of active protein C formed after 20 minutes was 1.4-fold increased after KLF2 overexpression compared with mock controls. TNF-α had no significant effect on protein-C activation with KLF2 overexpression, in contrast to a 3.5-fold reduction in mock-transduced or untreated cells. *P = .037. mOD indicates milli–optical density.