American Society of Hematology

Figure 1.

$Figure 1. Comparison of CD44act to other T-cell activation markers in total CD4 cells. (A) Naive peripheral lymph node T cells (1 × 106) were stimulated with 5 μg/mL plate-bound anti-CD3/anti-CD28, harvested at 8 and 24 hours, and compared with unactivated cells (T = 0). Cells were stained as indicated in conjunction with Fl-HA for FACS. Conversion to CD44act is late relative to changes in other markers and occurs only on a fraction of cells. (B) Line graph representation of the changes over time in expression of CD44act compared with other indicated activation markers. (C) Two-color flow cytometric analysis of DO11.10 transgenic T cells stimulated for 24 hours with 25 μM OVA peptide plus APCs. Fl-HA staining was done with and without the HA-blocking anti-CD44 mAb, KM81.$

Comparison of CD44^act to other T-cell activation markers in total CD4 cells. (A) Naive peripheral lymph node T cells (1 × 10⁶) were stimulated with 5 μg/mL plate-bound anti-CD3/anti-CD28, harvested at 8 and 24 hours, and compared with unactivated cells (T = 0). Cells were stained as indicated in conjunction with Fl-HA for FACS. Conversion to CD44^act is late relative to changes in other markers and occurs only on a fraction of cells. (B) Line graph representation of the changes over time in expression of CD44^act compared with other indicated activation markers. (C) Two-color flow cytometric analysis of DO11.10 transgenic T cells stimulated for 24 hours with 25 μM OVA peptide plus APCs. Fl-HA staining was done with and without the HA-blocking anti-CD44 mAb, KM81.

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