Effects of Cdc42GAP deletion on phenotypic HSP number and erythropoietic progenitor activities. (A) WT or homozygous E14.5 fetal liver (n = 20 for each group) cells were stained with lineage-FITC, Sca1-PE, and c-Kit-APC and were examined by flow cytometry for HSP number and percentage composition. (B-D) E14.5 fetal liver (n = 20 for each group) cells (B-C) or 3-day-old pup (n = 5 for each group)-derived bone marrow cells (D) were cultured in methylcellulose medium supplemented with 100 ng/mL SCF, 100 ng/mL IL-3, 4 U/mL EPO, and 100 ng/mL G-CSF for 7 days for the development of CFU-GM, BFU-E, or CFU-GEMM colonies (B) or with 100 ng/mL SCF and 4 U/mL EPO for 2 days for the growth of CFU-E colonies (C-D).