KSHV vGPCR expression inhibits lytic gene induction by TPA and butyrate. BC3.14 cells were treated for 48 hours with 2 μg/mL doxycycline where indicated to express vGPCR, after which cells were also exposed to butyrate (1 mM or 2 mM) (A) or TPA (20 ng/mL) (B) to induce KSHV lytic gene transcription. q RT-PCR was performed for ORF 50 and ORF 26 message. Results are normalized to untreated cells (lane 1). Shown in both panels are 2 independent experiments each done in duplicate. Error bars indicate SD. ^*Significant increase over baseline, P ≤ .05; ^**Significant decrease from maximum induction, P ≤ .05.