Cdk2 activity is necessary for full chemical induction of KSHV lytic genes. BC3 cells were infected with lentivirus expressing the Cdk2 dominant-negative coding sequence in either forward or reverse (control) orientation; 72 hours later, cells were treated with 1 mM butyrate for 48 hours as indicated. Quantitative RT-PCR was performed for ORF 50 (top), and ORF 26 (bottom). Figure represents 2 independent experiments, each done in duplicate. Error bars indicate SD. ^*Significant increase over baseline, P ≤ .05; ^**Significant decrease from maximum induction, P ≤ .05.