Putative AKT consensus phosphorylation site in GATA-1. (A-B) Alignment of the C-terminal zinc finger domain of GATA family of transcription factors. Highly conserved putative AKT consensus phosphorylation sequence surrounding S310 in hematopoietic GATAs (A) and among different species (B) are highlighted in black. Conserved cysteine residues in the C-terminal zinc finger domain are highlighted in gray. Arrow shows the highly conserved serine residue. (C) Schematic of wild-type (WT) GATA-1 protein. The serine (S) to alanine (A), aspartic acid (D), glutamic acid (E), and alanine to serine mutations in GATA-1 constructs are indicated. Nuclear localization sequence (NLS).