Figure 2.
Binding of 125I-CTRP-1 to fibrillar collagen type I. Plates were coated with fibrillar collagen type I and incubated with the indicated concentration of 125I-CTRP-1 in the absence (total binding, •) and presence (nonspecific binding, ○) of 250 μg/mL unlabeled CTRP-1. Specific binding (▴) was calculated as the difference between the total and nonspecific values. Each point represents the mean of duplicate wells and is representative of 4 separate experiments. Inset shows the Scatchard plot of the specific binding data. B indicates bound 125I-CTRP-1; F, free 125I-CTRP-1.
