American Society of Hematology

Figure 6.

$Figure 6. Effects of PPP on caspases. (A) Cleavage of caspase 8, 9, and 3 was analyzed by Western blotting of lysates obtained from serum-starved RPMI 8226 cells treated with 1 μM PPP for 2, 4, and 24 hours and 6.7 nM IGF-1 for 5 minutes at each time point. (B) RPMI 8226 cells were left untreated or were treated with PPP at 0.5 μM or the agonistic Fas-antibody CH11 (200 ng/mL) alone (filled area), or in the presence of the pan-caspase inhibitor Z-VAD-fmk at 30 μM (open area), for 48 hours. Two independent experiments were performed and one representative is shown. The bars indicate the apoptotic TUNEL-positive fraction.$

Effects of PPP on caspases. (A) Cleavage of caspase 8, 9, and 3 was analyzed by Western blotting of lysates obtained from serum-starved RPMI 8226 cells treated with 1 μM PPP for 2, 4, and 24 hours and 6.7 nM IGF-1 for 5 minutes at each time point. (B) RPMI 8226 cells were left untreated or were treated with PPP at 0.5 μM or the agonistic Fas-antibody CH11 (200 ng/mL) alone (filled area), or in the presence of the pan-caspase inhibitor Z-VAD-fmk at 30 μM (open area), for 48 hours. Two independent experiments were performed and one representative is shown. The bars indicate the apoptotic TUNEL-positive fraction.

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