Phenotype of TMTregs. T-cell transfer and vaccination were conducted as described in Figure 1. Spleen cells from vaccinated tumor-free and TM mice were stained with anti-CD4, anti-Thy1.1, and the indicated mAb. Dot plots shown are gated on CD4⁺Thy1.1⁺ donor cell population. Value shown in each plot is the percentage of the indicated population. The data shown are representative of 3 separate experiments with similar results. GITR indicates glucocorticoid-induced TNF receptor.