Figure 1.
Size fractionation of PEL cell lysates. Lysates from BC-3 (A) and BC-1 (B) cell lines were separated using gel filtration chromatography on a Superdex 200HR column. Fractions were resolved by SDS-PAGE (12%) and analyzed by Western blotting (WB) with antibodies against v-cyclin, CDK6, and p27KIP1. The elution profile of the molecular weight (Mw) standards is indicated on the top.